Immunostimulatory effects of aloe vera and β-glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs [Köpeklerde karma aşi{dotless} ile aşi{dotless}lama sonrasi{dotless} hücresel ve humoral i̇mmun cevaplar üzerinde aloe vera ve β-glucan'i{dotless}n i̇mmunostimülatör etkileri]

The aim of this study was to evaluate the effects of aloe vera and β-glucan on lymphocyte subsets,haematological parameters and immunglobulin concentrations following vaccination in dogs. for this purpose,totally 20 street dogs were used. the animals were allocated into five groups. group 1 consisted of only polyvalent vaccine applied dogs. group 2 and 3 were only aloe vera and β-glucan applied dogs,respectively. group 4 and 5 were consisted of polyvalent vaccine in addition to aloe vera and β-glucan applied dogs,respectively. blood samples were collected before vaccination and after vaccination (av). although platelet (plt) counts in group 1 decreased av,in all other groups (group 2,3,4,5) it increased. white blood cell (wbc) and peripheral blood mononuclear lymphocyte (pbml) counts (in all groups),peripheral blood poly-morphonuclear lymphocyte (pbpl) counts (group 1,2,4,5),neutrophil (group 1,4,5),monocyte (group 2,3,5),packed cell volume (pcv) ratios (group 2 and 3) and haemoglobin (hgb) concentrations (group 3) increased av. but,lymphocyte ratios in all groups did not change av. cd8 t lymphocyte ratios in all groups increased at 7th and 14th days av. although cd3 t lymphocyte ratios in group 1 increased at 7th day av,cd4 t and b lymphocytes ratios did not change av period. but,both cd3 and cd4 t lymphocytes ratios in all other groups increased at 7th and 14th days av. moreover,b lymphocytes ratios in all groups except for group 1 increased av. on the other hand,b lymphocytes ratios in group 2 and 3 increased only 7th day av,while this increase in group 4 and 5 were present on both 7th and 14th days av. serum igm concentrations in group 1 increased only 7th day av,whereas its levels did not change in group 2 and 3. serum igg concentrations in group 1,2 and 3 increased only 14th day av. but,serum igm and igg concentrations in group 4 and 5 increased at 7th and 14th days av. administration of aloe vera and β-glucan after vaccination in dogs may stimulate both cellular and humoral immun responses. additionally,it might have restorative effect on plt counts.