Hsp-70 gene expression analyses in the different ages of rainbow trout [Farkli{dotless} yaşlardaki gökkuşaǧi{dotless} alabali{dotless}klari{dotless}nda (Oncorhynchus mykiss) Hsp-70 geninin ekspresyon analizi]

The purpose of this study was to determinate the association between heat shock protein 70 (hsp-70) expression and the age in rainbow trout. total rna was extracted with trizol reagent (life technologies) and the first strand cdna was synthesized using super script iii reverse transcriptase. gene amplification was done by pcr method and quantitative determination of gene expression was using beta actin gene as control. consequently,we demonstrated that hsp70 expression significantly increased the age.