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Detection of Cryptosporidium spp. in humans and calves through nested PCR and carbol fuchsin staining methods in Ankara,Turkey [Ankara'da i̇nsan ve buzaǧi{dotless}larda Cryptosporidium spp. Varli{dotless}ǧi{dotless}ni{dotless}n nested PCR ve carbol fuchsin boyama yöntemleri ile belirlenmesi]
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نویسنده
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sakarya y. ,kar s. ,tanyuksel m. ,karaer z. ,babur c. ,vatansever z.
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منبع
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journal of the faculty of veterinary medicine, kafkas university - 2010 - دوره : 16 - شماره : 6 - صفحه:977 -980
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چکیده
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In this study,a total of 130 fecal samples,obtained from 98 humans and 32 calves from different hospitals and dairy farms in ankara,respectively were analyzed with carbol fuchsin staining and nested pcr to determine the presence of cryptosporidium spp. nested pcr revealed a total of 13 (10.0%) positive amplification results; 12 (37.5%) were from calves and 1 (1.02%) from humans. oocysts were observed in 8 (6.15%) fecal samples belonging to 7 (21.88%) calves and 1 (1.02%) human by carbol fuchsin staining. concerning the detection technics,it was concluded that nested pcr can be successfully used in diagnosis of cryptosporidiosis,and although carbol fuchsin staining method has sufficient specificity it is less sensitive,especially in cases characterized with excretion of fewer oocysts.
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کلیدواژه
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Ankara; Carbol fuchsin; Cryptosporidium; Nested PCR
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آدرس
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ankara university,faculty of veterinary medicine,department of parasitology,06110 dişkapi, Turkey, namik kemal university,department of biology, Turkey, gulhane military medical academy,department of medical parasitology, Turkey, ankara university,faculty of veterinary medicine,department of parasitology,06110 dişkapi, Turkey, the ministry of health refik saydam hifzissihha centre,department of parasitology, Turkey, kafkas university,faculty of veterinary medicine,department of parasitology, Turkey
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Authors
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