Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography [Yüksek performansli{dotless} si{dotless}vi{dotless} kromatografisi kullanarak tavşan plazmasi{dotless}nda i̇buprofenin tayini]

The aim of the present study was to develop and validate a procedure based on high-performance liquid chromatography (hplc) for determination of ibuprofen in rabbit plasma. separation of ibuprofen and naproxen (internal standard,is) was achieved on an ace c18 column (5 μm,250×4.6 mm i.d.) using uv detection with λ=225 nm. the mobile phase consisted of 20 mm phosphate buffer (ph 7) containing 0.1% trifluoroacetic acid (tfa)-acetonitrile (65:35,v/v). the analysis was performed in less than 10 min with a flow rate of 1 ml/min. excellent linearity was found between 0.5 and 40 μg/ml. intra- and inter-day precision values for ibuprofen in plasma were less than 4.97,and accuracy (relative error) was better than 7.20%. the recoveries for all samples were >92.8%. the limits of detection (lod) and quantification (loq) of ibuprofen were 0.10 and 0.25 μg/ml,respectively. the described hplc method has adequate sensitivity and specificity to study pharmacokinetics of ibuprofen in rabbits,and could be adapted also to clinical pharmacokinetic study.