Immunostimulating effect of levamisole on spleen and head-kidney leucocytes of rainbow trout (Oncorhynchus mykiss,Walbaum 1792) [Levamisolün gökkuşaǧi{dotless} alabali{dotless}ǧi{dotless}ni{dotless}n (Oncorhynchus mykiss,Walbaum 1792) dalak ve ön böbrek lökositlerine i̇mmunostimulatik etkisinin araşti{dotless}ri{dotless}lmasi{dotless}]

An in vitro cultivation method for spleen and head kidney organ section were used to investigate the effects of levamisole on the immune response. after 10 days of culture with 10 μg/ml,50 μg/ml and 100 μg/ml or no levamisole in the culture media,the non specific defense reaction were measured by glass-adherent nbt (nitroblue tetrazolium) positive cells activation and phagocytic activity. elevation in phagocytic activity was found for 10 μg/ml and 50 μg/ml levels of levamisole (p<0.001),but no differences were found at levels of phagocytic activity in between 100 μg/ml levamisole groups and control groups (p>0.05). in the spleen and head kidney leucocytes,increased response to levamisole was shawn for all doses in the glass-adherent nbt positive cells activation.