Investigation of poly-β-hydroxybutyrate (PHB) production by Bacillus subtilis ATCC 6633 Under different conditions [Farkli{dotless} koşullar alti{dotless}nda Bacillus subtilis ATCC 6633 tarafi{dotless}ndan poli-β-hidroksibütirat (PHB) üretiminin araşti{dotless}ri{dotless}lmasi{dotless}]

The aim of our study was to assess the phb productivity of bacillus subtilis atcc 6633 strain under different conditions. phb amount was measured by uv-vis spectrophotometer at 235 nm. the effects of the incubation time,temperature,ph,carbon sources,nitrogen sources and the rate of carbon/nitrogen ratio on phb production rate were investigated. the results demonstrated that the highest phb production was obtained after 24 h of incubation (10.4981 μg/ml),at 7.0 of ph (10.4981 μg/ml),with d-mannitole (23.6623 μg/ml) as carbon source,l-glycine (14.6217 μg/ml) as nitrogen source,c/n ratio of 2.5 (3.2481 μg/ml) and with a temperature of 30°c in culture media (10.4981 μg/ml).