Detection of Enterohemolysin and Intimin Genes in Escherichia coli O157:H7 Strains Isolated from Calves and Cattle in Afyonkarahisar - Turkey [Afyonkarahisar'da si{dotless}ǧi{dotless}r ve buzaǧi{dotless}lardan izole edilen escherichia coli o157:H7 suşlari{dotless}nda i̇ntimin ve enterohemolizin genlerinin belirlenmesi]

The aim of this study was to detect enterohemolysin (ehlya) and intimin (eaea) virulence genes of 14 escherichia coli o157:h7 strains isolated from 457 fecal samples (237 calves and 220 cattle) by pcr. while ehlya gene was determined in 13 (92.8%) strains,the eaea gene was positive in 8 (57.1%) strains. of the 8 eaea genes,4 (50.0%) were obtained from diarrheic calves,2 (25.0%) from non-diarrheic calves,and 2 (25.0%) from healthy cattle. a total of 7 (50.0%) strains were determined to harbour both of the ehlya and eaea genes. this study confirmed that calves,especially diarrheic,and cattle are a reservoir of e. coli o157:h7 strains that may be pathogenic for human.