Mitochondrial DNA D-loop and 12S regions analysis of the long-crowing local breed Denizli fowl from Turkey [Türkiye uzun ötüşlü yerli irki{dotless} Denizli tavuǧunun mitokondriyal DNA D-loop ve 12s bölgeleri analizi]

Aim of this study is to analyze the genetic structure of long-crowing denizli chicken,a turkish local fowl,using nucleotide sequences of the mitochondrial dna 12s and d-loop regions. dna isolation was carried out using feather samples of cocks of this local breed. d-loop and 12s regions were amplified using polymerase chain reaction technique and ca 1230 bp and 950 pb pcr products were obtained respectively. native genotype denizli fowl's d-loop and 12s regions were sequenced and sequencing data were analyzed and compared with the related published data. nucleotide content of the 12s region showed a 32% a,19% t,19% g and 30% c,whilst at and gc ratios were found as 59.88% and 40.12% respectively for d-loop region. d-loop sequence data analysis clearly identified the denizli fowl within the clade of long-crowing cocks. the results give the first informative data about the mitochondrial dna d-loop and 12s regions of this local breed from turkey.