Identification of β-lactoglobulin gene SacII polymorphism in honamli,hair and saanen goat breeds reared in burdur vicinity [Burdur i̇li ve civari{dotless}nda yetiştirilen honamli{dotless},ki{dotless}l ve saanen keçi irklari{dotless}nda β-lactoglobulin geni SacII polimorfizminin belirlenmesi]

This study was conducted to determine dna-polymorphism of a sacii rflp at exon 7 of the β-lactoglobulin (β-lg) gene in honamli (31),hair (39) and saanen (41) goat breeds. a total of 111 goats were genotyped for the β-lactoglobulin-sacii polymorphism by polymerase chain reaction and restriction fragment length polymorphism (pcr-rflp). in the studied breeds,digestion of amplification product with sacii restriction enzyme revealed two alleles namely,a and b and three genotypes (aa,bb and ab). allelic frequencies for hair,saanen and honamli breeds were 0.42,0.37 and 0.53 respectively for a allele; 0.58,0.63 and 0.47 respectively for b allele,while genotypic frequencies were 0.13,0.12 and 0.19 for aa,0.28,0.39 and 0.13 for bb,and 0.59,0.49 and 0.68 for ab respectively. deviation from hardy-weinberg equilibrium was not observed in the studied breeds. as a result,this study provided information on the polymorphism of β-lactoglobulin in three goat breeds. additionally,this study reported the existence of a genetic polymorphism at β-lg gene in honamli goat breed for the first time.