The effect of different storage temperature on sperm parameters and DNA damage in liquid stored New Zealand rabbit spermatozoa [Farkli{dotless} saklama isi{dotless}lari{dotless}ni{dotless}n ki{dotless}sa süreli saklanan yeni zelanda tavşan spermatozoasi{dotless}ni{dotless}n DNA hasari{dotless} ve sperm parametreleri üzerine etkisi]

The effect of two different temperatures (4°c and 15°c) on motility,plasma membrane integrity,acrosome abnormality and dna damage of rabbit spermatozoa was evaluated at 0 and 24 h of liquid storage. ejaculates collected from six new zealand male rabbits by artificial vagina and pooled at 37°c following evaluation. pooled ejaculate was divided into two equal aliquots and diluted with the tris based semen extender at a final concentration of approximately 40x10 6 sperms/ml in a eppendorf plastic tube. there were no significant differences in the percentage of above mentioned parameters between 4°c or 15°c at the beginning of liquid storage (0 h). the percentages of motility (75.0±1.83%) and plasma membrane functional integrity (71.2±1.14%) at 15°c was significantly better than that of liquid stored semen at 4°c (67.9±1.01% and 65.3±1.38%,p<0.05,respectively) at 24 h of storage. the percentage of acrosome abnormality at 24 h wasn't affected by the different storage temperature. the influence of storage temperature and the length of time on spermatozoa dna damage was found statistically significant (p<0.001). the storage period for up to 24 h lead to an increase in the percentage of spermatozoa dna damage (p<0.001). the percentages of dna damage at 4°c was statistically higher than 15oc (p<0.001). in conclusion,15°c may be prefered when liquid stored rabbit semen are used for 24 h.