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The effect of different storage temperature on sperm parameters and DNA damage in liquid stored New Zealand rabbit spermatozoa [Farkli{dotless} saklama isi{dotless}lari{dotless}ni{dotless}n ki{dotless}sa süreli saklanan yeni zelanda tavşan spermatozoasi{dotless}ni{dotless}n DNA hasari{dotless} ve sperm parametreleri üzerine etkisi]
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نویسنده
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sariözkan s. ,cantürk f. ,yay a. ,akçay a.
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منبع
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journal of the faculty of veterinary medicine, kafkas university - 2012 - دوره : 18 - شماره : 3 - صفحه:475 -480
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چکیده
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The effect of two different temperatures (4°c and 15°c) on motility,plasma membrane integrity,acrosome abnormality and dna damage of rabbit spermatozoa was evaluated at 0 and 24 h of liquid storage. ejaculates collected from six new zealand male rabbits by artificial vagina and pooled at 37°c following evaluation. pooled ejaculate was divided into two equal aliquots and diluted with the tris based semen extender at a final concentration of approximately 40x10 6 sperms/ml in a eppendorf plastic tube. there were no significant differences in the percentage of above mentioned parameters between 4°c or 15°c at the beginning of liquid storage (0 h). the percentages of motility (75.0±1.83%) and plasma membrane functional integrity (71.2±1.14%) at 15°c was significantly better than that of liquid stored semen at 4°c (67.9±1.01% and 65.3±1.38%,p<0.05,respectively) at 24 h of storage. the percentage of acrosome abnormality at 24 h wasn't affected by the different storage temperature. the influence of storage temperature and the length of time on spermatozoa dna damage was found statistically significant (p<0.001). the storage period for up to 24 h lead to an increase in the percentage of spermatozoa dna damage (p<0.001). the percentages of dna damage at 4°c was statistically higher than 15oc (p<0.001). in conclusion,15°c may be prefered when liquid stored rabbit semen are used for 24 h.
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کلیدواژه
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Comet assay; DNA integrity; Liquid storage; Rabbit; Semen
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آدرس
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erciyes university,faculty of veterinary medicine,department of reproduction and artificial insemination, Turkey, erciyes university,faculty of medicine,department of biophysics, Turkey, erciyes university,faculty of medicine,department of histology and embryology, Turkey, erciyes university,faculty of veterinary medicine,department of biostatistics, Turkey
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Authors
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