Genetic variability of CSN1S1 gene in goat populations raised in southeastern region of Turkey [Güneydoǧu anadolu bölgesi'nde yetiştirilen keçilerde alfa-s1-kazein (CSN1S1) genindeki genetik çeşitlilik]

The objective of this study was to investigate genetic variability of csn1s1 gene coding for alpha-s1-casein in goat populations raised in southeastern region of turkey. blood samples were collected from goats raised in kilis(n=60),sanliurfa (n=56),and siirt (n=55) provinces of turkey. from the blood samples dna was isolated by using phenol-chloroform extraction. genotypes of animals were determined by using polymerase chain reaction (pcr),allele specific pcr or pcr and restriction fragment length polymorphism methods. in kilis and sanliurfa populations csn1s1 a*,b*,f and n alleles were observed,while in siirt population only a* and b* alleles were found. frequencies of a*,b*,f and n alleles were 0.375,0.367,0.017 and 0.242 in kilis,0.632,0.208,0.009 and 0.151in sanliurfa and 0.782,0.218,0.000 and 0.000 in siirt populations,respectively. csn1s1 e and 01 alleles were not observed among the populations studied. observed and expected genotype frequencies did not differ significantly (p>0.05). the results of this study suggested that there were sufficient genetic variability of csn1s1 gene especially in sanliurfa and kilis populations in order to select individuals for different breeding purposes.