Characterization of calpastatin gene in purebred and crossbred turkish grey steppe cattle [Safkan ve melez Türk boz irk si{dotless}ǧi{dotless}rlarda Kalpastatin geninin Karakterizasyonu]

Calpastatin (cast) is a specific inhibitor of calcium-dependent neutral protease μ-calpain found in mammalian tissues. the genetic variants in the bovine cast gene were analysed by using the polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) method. the animal material of the study consisted of 132 bulls and heifers in turkish grey steppe cattle and turkish grey steppe x brown swiss crossbreds. c and g alleles are characterized by cast/rsai polymorphism were detected in the sample of animals were studied. allele frequency c was significantly frequent in the crossbred group compared to the pure turkish grey steppe animals (p<0.05). in the total samples of animals,the average allele frequency c was 56.1%. genotypic frequencies were estimated as 0.257,0.499 and 0.243 in the purebred turkish grey steppe,and 0.388,0.470 and 0.142 in the turkish grey steppe x brown swiss crossbred population for genotypes cc,cg and gg,respectively. as a result,genotypic distributions were equilibrium in both pure and crossbred examples of turkish grey steppe cattle.