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Capsular typing and antimicrobial susceptibility of Pasteurella multocida isolated from different hosts [Farkli{dotless} hayvan türlerinden i̇zole edilen Pasteurella multocida suşlari{dotless}ni{dotless}n kapsüler tiplendirilmesi ve antimikrobiyal duyarli{dotless}li{dotless}klari{dotless}]
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نویسنده
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güler l. ,gündüz k. ,sarişahin a.s.
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منبع
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journal of the faculty of veterinary medicine, kafkas university - 2013 - دوره : 19 - شماره : 5 - صفحه:843 -849
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چکیده
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In this study,75 pasteurella multocida isolates from diferent animal species of cattle,sheep,goat and rabbit isolated during 2001-2012 were identified and characterized by multiplex pcr for 5 capsular types of a,b,d,e,and f. thirty six of isolates were isolated from cattle (19 lungs,12 nasal swabs and 5 milk samples),33 from sheep lungs,3 from goat lungs and 3 from rabbit lungs. each isolates was obtained from diferent herds/focks except 9 bovine nasal swab isolates (6 and 3),which were from two herds. all p. multocida isolates identified by conventional methods were confirmed by multiplex pcr. thirty three (91.6%) of 36 bovine isolates were serogroup a; 11 (33.3%) of 33 sheep isolates were serogroup d,2 (6.0%) were serogroup a,1 (3.0%) was serogroup f and 19 (57.5%) were untypable; 2 of 3 goat isolates were serogroup d. all of 3 rabbit isolates were untypable. overall 26 (34.6%) isolates were untypable. no strain was detected from serogroup b and e. antimicrobial susceptibility test results of 50 p. multocida strains using disk difusion test showed that all strains were susceptible to ceftiofur,enrofoxacin,forfenicol and trimethoprim-sulfamethoxazole. six (12.0%) strains were resistant to spectinomycin,6 (12.0%) to tetracycline,3 (6.0%) to tilmicosin and 3 (6.0%) to erythromycin. multiple resistance was detected in 7 (4 tetracycline+spectinomycin,2 tilmicosin+erythromycin,1 tetracycline + tilmicosin + erythromycin) of 11 resistant strains isolated from 10 cattle and 1 sheep.
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کلیدواژه
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Antimicrobial susceptibility; Capsular typing; Cattle; Pasteurella multocida; PCR; Sheep
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آدرس
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department of microbiology,mehmet akif ersoy university, Turkey, konya veterinary control institute,tr-42080 meram, Turkey, konya veterinary control institute,tr-42080 meram, Turkey
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Authors
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