Molecular characteristics of Staphylococcus aureus isolates from buffaloes milk [Manda sütünden İzole edilen Staphylococcus aureus türlerinin moleküler karakterizasyonu]

The aim of the present study was to analyse the aroa and x region of spa genes on staphylococcus aureus isolates collected from milks of native buffaloes in north-west of iran. for this purpose,seventy five s. aureus isolates were examined by pcr-rflp. amplification of aroa gene revealed a single amplicon with a size of approximately 1,153 bp,but restriction profile analysis of this amplicon yielded three different genotypes including genotype a (9.3%),b (88%) and n (2.7%). amplification of x region of the spa gene showed an amplicon of 1110; 1130; 1160; 1190 bp in 19,15,12 and 29 isolates,respectively. hhai digestion of this amplicons demonstrated that the isolates having the same pcr band size were in the same pcr-rflp patterns and there was not variation into isolates belonged to the same pcr amplicon.