Can sequential human embryo culture media be used in bovine in vitro embryo culture? [Ardi{dotless}şi{dotless}k İnsan embriyo kültür medyumu si{dotless}ǧi{dotless}r embriyolari{dotless}ni{dotless}n in vitro kültüründe kullani{dotless}labilir mi?]

The aim of the study was to investigate potential use of sequential human embryo culture media in culture of bovine embryos. bovine oocytes were matured in tissue culture medium-199 (tcm-199) for 22 h at 38.5°c and fertilized in modified tyrode-albumine-lactate-pyruvate medium (mtalp). the putative zygotes were randomly allocated to two embryo culture media groups; (1) synthetic oviduct fluid (sof) supplemented with fatty-acid free bovine serum albumin (faf-bsa) (8 mg/ml) and (2) sequential human embryo culture media [quinn's advantage medium-(qam)] supplemented with faf-bsa (8 mg/ml). zygotes were cultured in sof and sequential qam for 9 days (5% co2,5% o2,and 90% n2) at 38.5°c. cleavage (73.3% and 72.2%),morula (37.6% and 33.2%) and blastocysts rates (23.9% and 22.9%) were similar among groups (p>0.05),but the total blastocyst cell number were significantly higher in blastocysts developed in sof (101.6±4.0) than those in sequential qam (87.4±3.2) (p<0.05). qam may be suggested to use in culture as an alternative media in terms of supporting embryo development,but low cell number in blastocysts produced in qam may suggest a possible low pregnancy rate.