Genetic characterization of Turkish cattle breeds by microsatellite markers: Usefulness for parentage testing [Türkiye yerli si{dotless}ǧi{dotless}r irklari{dotless}ni{dotless}n mikrosatellit belirteçler ile genetik karakterizasyonu: Kimliklendirme çali{dotless}şmalari{dotless}nda kullani{dotless}labilirliǧi]

Objective of this study was to evaluate microsatellite markers in paternity testing of native cattle breeds in turkey. blood samples were collected from anatolian black (n=51),anatolian grey (n=54),south anatolian red (n=51),native southern anatolian yellow (n=51),east anatolian red (n=45) and zavot (n = 19) cattle. from the blood samples dna was isolated by using a standard phenol/chloroform method. a total of 20 microsatellite loci were selected from a fao/isag-suggested list. polymerase chain reaction products were separated by capillary electrophoresis and marker genotypes were determined by fragment analysis. in statistical analyses,allel numbers,observed (ho) and expected (he) heterozygosities,deviation from hardy-weinberg equilibrium and probability of exclusion (pe) at each microsatellite locus were calculated. a total of 269 different alleles were observed and the mean allele was identified as 13.45. mean ho and he values were observed as 0.619-0.852 and 0.669-0.877,respectively. the results indicated that the microsatellite test panel including the most informative 7 loci had total pe value of >0.9999 in each populations and can thereby be used for parentage testing studies of native cattle breeds in turkey.