Effects of nonylphenol on motion kinetics,acrosome and mitochondrial membrane potential in frozen-thawed bull sperm [Nonylphenol'ün dondurulmuş-çözdürülmüş boǧa sperma hareket kinetiǧine,akrozom ve mitokondrial membran potansiyeline etkisi]

Recent reports have shown that the nonylphenol (np),a well-known toxic environmental chemical has adverse effects on sperm in different species. therefore,the current study was aimed to determine the deleterious effect of np on frozen-thawed bull sperm. sperm were exposed to different concentrations of np (1,10,100,250 and 500 μg np/ml) dissolved either in dmso or ethanol and incubated for 4 h at 37°c. sperm parameters were assessed at 0,1st,2nd,3rd,and 4th h of incubation. both percent motility and mitochondrial membrane potential (mmp) of sperm have decreased significantly (p<0.05) at dose level of ≥ 250 μg np/ml. acrosome reaction was induced (p<0.05) even at minimal dose concentrations of 1μg np/ml (p<0.05). however,the percentage of acrosome reacted sperm increased (p<0.05) with increase in dose level. in conclusion,np impairs the sperm characteristics by adversely affecting motion kinetics,mitochondrial membrane potential and inducing premature acrosome reaction in bovine.