Biochemical profile of paenibacillus larvae repetitive element polymerase chain reaction (rep-PCR) genotypes in Bulgaria [Bulgaristanda paenibacillus larvae repetitive element polymerase chain reaction (rep-PCR) genotiplerinin biyokimyasal Profili]

The aim of the present study was to determine the biochemical profile of paenibacillus larvae repetitive element polymerase chain reaction (rep-pcr) genotypes in bulgaria and to assess the link between genotype and phenotype. a total of 103 isolates (genotype ab,n=21; genotype ab,n=82) and a reference strain nbimcc 8478 were analyzed using identification system biolog gen iii and nitrate reducing ability. genotypes ab and ab showed a particular metabolic fingerprint based on 71 carbon sources provided by biolog system. considering the nitrate reducing ability,mannitol and salicin utilization,the strains were distributed into biotypes i,iii,iv and viii. the majority of paenibacillus larvae ab clustered into biotype iii while ab were grouped mainly into biotype i. biotypes i and iv were not found among the tested ab strains. this study showed the obvious link between rep-pcr genotypes ab/ab and biochemical phenotype that can be useful in epidemiologic situations to trace the source of infection and to control the disease.