Determining gene expression profile of GPX 1 in the liver of diabetic rats treated with capsaicin by real-time PCR [Kapsaisin uygulanan diabetik sıçanların karaciğer dokusunda GPX 1’in real time-PCR ile expresyon profilinin çıkarılması]

The purpose of the present study was to determine the glutathione peroxidase 1 (gpx 1) gene expression by real time pcr in the liver of healthy and diabetic rats treated with capsaicin. twenty sprague-dawley rats were used in our study. rats were divided into four groups: group i: diabetic rats (n=5),group ii: capsaicin injected rats (n=5),group iii: capsaicin injected diabetic rats (n=5),and group iv: control rats (n=5). capsaicin injection began 72 h after streptozotocin (stz) injection. capsaicin (1 mg/kg) was prepared in 10% ethanol,1% tween 20,and 80% sterile water and subcutaneously injected daily in both groups ii and iii for two weeks. the results of rt-pcr conducted to determine gpx 1 gene expression indicated that capsaicin treated diabetic rats had higher gpx 1 expression compared to all groups including control (p<0.05). as a result,capsaicin causes an increase in gpx 1 gene expression at transcription level in the diabetic rat liver. further investigation is needed to determine if such an increase occurs at protein level using various methods such as western-blot analysis. © 2015,kafkas university. all rights reserved.