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Determination of helicobacter infections with 14C urea breath test (14C UBT) and polymerase chain reaction (PCR) in dogs and treatment [Köpeklerde Helikobakter Enfeksiyonlarının C14 Üre Nefes Testi (C14 UNT) ve Polimeraz Zincir Reaksiyonu (PZR) ile Belirlenmesi ve Tedavisi]
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نویسنده
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meral y. ,gökalp g. ,uçak semirgin s. ,ünlü söğüt m.
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منبع
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journal of the faculty of veterinary medicine, kafkas university - 2015 - دوره : 21 - شماره : 3 - صفحه:327 -332
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چکیده
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It was aimed to determine that if14c ubt (14c urea breath test) application as a “gold standard” in the human practice for diagnosing helicobacter infections could also be used in an easy,practical,and a reliable method in the veterinary practice. three groups were allocated in the study. dogs (n=41) were detected as clinically healthy in their physical examinations were grouped as healthy (group i). dogs (n=32) had clinical symptoms of vomiting after feeding,anorexia,epigastric pain during abdominal palpation were grouped as diseased and these diseased dogs (group ii) that were treated grouped as treatment (n=32) (group iii). helicobacter staining,polymerase chain reaction (pcr) and14c ubt were compared for diagnosis of helicobacteriosis before and after therapy. based on the results of the present study14c ubt was found 2 times more reliable when compared to pcr and 65 times more reliable than helicobacter staining technique. sensitivity for14c ubt,pcr and helicobacter staining technique was found as 96.55%,93.75%,and 59.38%,respectively. moreover specificity of the14c ubt,pcr and helicobacter staining technique was detected as 97.73%,97.56% and 92.68%,respectively. consequently,it was thought that14c ubt test could be used as a reliable method in veterinary practice. © 2015,kafkas university. all rights received.
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کلیدواژه
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14C Urea breath test; Diff-quick staining; Dog; Helicobacter spp; PCR
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آدرس
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university of ondokuz mayis,department of internal medicine,atakum, Turkey, university of ondokuz mayis,department of internal medicine,atakum, Turkey, university of ondokuz mayis,department of nuclear medicine,atakum, Turkey, university of ondokuz mayis,health school,atakum, Turkey
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Authors
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