Effects of various cryoprotective agents on post-thaw drone semen quality [Farklı kriyoprotektanların eritme sonrası arı sperm kalitesi üzerine etkileri]

The aim of the present study was to evaluate the effect of different cryoprotectants on post-thaw semen motility and plasma membrane integrity of drone spermatozoa. semen was obtained from mature drones (16 days or older) harvested from four colonies. collected semen was diluted to a final concentration of 1/5 (semen/extender) in 0% cryoprotectant (control),6% glycerol,6% ethylene glycol,6% 1,2 propanediol or 6% dmso using a two-step dilution method. the equilibrated semen was frozen in 0.25-ml straws. the percentage of sperm motility and swollen tails (host) spermatozoa were evaluated following dilution with extender a (non-cryoprotectant),equilibration and post-thaw stages. in terms of post-thaw motility and plasma membrane integrity recovery we can rank the used cryoprotectant as dmso,ethylene glycol,glycerol and 1,2 propanediol; respectively. in conclusion,post-thaw sperm motility and plasma membrane integrity of the present study was significantly better when sperm was frozen in dmso with respect to control,glycerol,ethylene glycol,1,2 propanediol (p<0.05). © 2015,veteriner fakultesi dergisi. all rights reserved.