In vitro evaluation of short-term preserved stallion semen in diferent doses and temperatures [Farklı doz ve sıcaklıklarda kısa süreli saklanan aygır spermasının]

This study was conducted to evaluate the efects of diferent temperatures and doses on stallion sperm survival after 24 h and 48 h short-term storage. ejaculates from four warm-blooded stallions were used in this study. ejaculates were collected by an open-ended artificial vagina which separates fractions of semen into sterile plastic cups. only the second and third fractions (spermatozoa-rich fractions) were used to obtain spermatozoa for the experiment. semen was diluted with inra 96 extender to final concentrations of 50,100,200 and 400x106sp/ml. diluted semen were stored at diferent temperatures (2,4 or 8°c) for 24-48 h. after 24 h and 48 h cooled storage,spermatozoon motility,abnormal spermatozoon ratio and membrane integrity were evaluated. according to results the highest motility values (52.5% and 55%) were determined at 50 and 100 x106sp/ml concentration at 4°c after 24 h cooled storage. after 48 h of storage,the highest motility was obtained from semen stored at 8°c. abnormal spermatozoon and membrane integrity values were not significantly diferent after cooled storage. consequently,it was determined that semen with 50 and 100 million spermatozoa/ml concentration stored at 4°c for 24 h and semen with 50,100,200,400 million spermatozoa/ml concentration stored at 8°c for 48 h gave better results with respect to motility than the others. © 2015,veteriner fakultesi dergisi. all rights reserved.