Protection and efficacy of cell culture propagated montanide adjuvant based inactivated vaccine against hydropericardium syndrome in poultry [Kümes Hayvanlarında Hidroperikardium Sendromuna Karşı Hücre Kültüründe Üretilmiş Montanide Adjuvant Bazlı İnaktive Edilmiş Aşının Koruyucu Etkisi]

The present study was designed to propagate field isolate of fowl adenovirus-4 in chicken embryo liver (cel) cell culture for development of efficient cell culture based inactivated vaccine. a pathogenic field isolate of fowl adenovirus-4 was propagated in chicken embryo liver cell culture. the liver homogenate virus (1030tcid50/ml) and cell culture propagated fav-4 (1030tcid50/ml) was used for preparing water based and oil based inactivated vaccines. the post-vaccination antibody response to all vaccines was tested by enzyme linked immunosorbant assay (till 3 week post vaccination) and the chickens were subjected to challenge protection studies. the groups injected with oil base (montanide) and water based conventional liver homogenate vaccines showed an average s/p (sample/positive) value of 0.341 and 0.323 respectively,the groups given p-1 cell culture passaged oil (montanide) based and water based vaccines showed an average s/p ratio 0.989 and 0.800 respectively. cell culture based montanide adjuvanted vaccine showed highest antibody response (s/p) among all groups. cell culture passaged vaccinated groups survived and provided 100% protection against challenge. liver homogenate based vaccines provided 80% protection. as a whole the cell culture passaged vaccines qualified the known standards of safety,sterility and potency. © 2015,veteriner fakultesi dergisi. all rights reserved.