Occurrence of ESBL-producing enterobacteriaceae in cubed beef samples [Kuşbaşı dana etlerinde GSBL-üreten enterobacteriaceae suşlarının varlıkları]

Extended spectrum beta-lactamase (esbl)-producing enterobacteriaceae is becoming a worldwide concern for the public health. they can adversely affect the treatment based on modern beta-lactam antibiotics against bacterial infections as well as possibly becoming a source for the spread of esbl-encoding genes among different and/or the same bacterial species. the objective of this study was to determine the occurrence of esbl-producing enterobacteriaceae from a total of 110 cubed beef samples sold in istanbul. the samples were initially homogenized in enterobacteriaceae broth. subsequently,the homogenized suspensions were exposed to pre-enrichment at 37°c for 18-24 h. after that,chromogenic esbl agar was used for selective enrichment at 37°c for 18-24 h again. the presumptive esbl-producers were sub-cultured on trypton soy agar,followed by an overnight incubation at 37°c. the isolates were identified by vitek®ms (biomérieux). finally,the identified isolates were subjected to agar disc diffusion testing,disc diffusion confirmation testing and mic determination testing using a combination of cefotaxime (ctx),ceftazidime (caz),and cefpodoxime (cpd) discs ± clavulanic acid (cv) according to the guidelines by clinical and laboratory standards institute. the results revealed that a total of 23 isolates were confirmed to be positive for esbl-production. of 23 isolates,the most common species was determined as e. coli (30%),followed by c. brakii (22%),e. cloacae (17%),k. pneumoniae (9%),c. freundii (9%),s. fonticola (4%),k. intermedia (4%),and m. wisconsensis (4%). in conclusion,the results of this study provided that the beef samples harbored esbl-producing enterobacteriaceae,and they may,therefore,hold a potential risk for the colonization of the consumers with esbl-producers. © 2016 veteriner fakultesi dergisi. all rights reserved.