RelA/p65-mediated innate immune response affecting ndv replication in CEF [CEF’teki NDV replikasyonunu etkileyen relA/p65-güdümlü doğal ımmun tepki]

Newcastle disease virus (ndv) is a non-segmented and single-stranded negative-sense rna (-)ssrna virus. previous studies indicate that ndv elicit hosts to induce strongly innate immune responses. the virus replication can cause hosts to express more type i interferon (ifn) via nuclear kappa b (nf-kb) pathways and further activate ifn-stimulated genes (isgs) to retard virus growth and induce cells for developing specific protection. this study investigated the role of rela/p65 in innate immune responses against ndv replication by using the virus infection chicken embryo fibroblast cells (cef). the result showed that the mrna and protein levels of rela/p65 increased markedly in cef infected with strains of ndv which differed in their virulence,and rela/p65 translocated from the cytoplasm to the nucleus. further research indicated that specific sirna could inhibit expression of rela/p65,accordingly,mrna levels of ifn-α,ifn-β and stat1 decreased significantly and the replication kinetics of 2 ndvs were enhanced after inhibition of rela/p65. in conclusion,cef can synthesize more type i ifn via rela/p65 pathways after ndv infection,which retards ndv replication and spreads in the early phase of viral infection. © 2016,veteriner fakultesi dergisi. all rights reserved.