Identification of genetic variation of melatonin receptor 1A (MTNR1A) gene in kıvırcık breed ewes by Mnli and rsai restriction enzymes [Kıvırcık irkı koyunlarda melatonin reseptör 1A (MTNR1A) geninin mnlI ve rsai restriksiyon enzimleri ile genetik varyasyonunun belirlenmesi]

Melatonin receptor 1a (mtnr1a) gene encodes melatonin hormone which regulates the function of seasonal reproductive activity in sheep. the aim of this study was to make the genetic characterization and identify the variant alleles of mtnr1a gene in kıvırcık breed. blood samples of 110 kıvırcık sheep were collected from five different farms located in kırklareli and istanbul. dna extraction was performed from blood samples. exon 2,the polymorphic region of melatonin receptor 1a gene,was amplified and pcr products were genotyped by using mnli and rsai enzymes. the observed alleles and genotypes for mnli enzyme were; m (0.891),m (0.109) and mm (0.782),mm (0.218) respectively. kıvırcık sheep was null from mm genotype. also identified alleles were c (0.682),t (0.318) and genotypes were cc (0.582),ct (0.200),tt (0.218) for rsai enzyme. the most frequent genotypes were mm (78%) and cc (58%) in kıvırcık ewes. since mm and cc genotypes were known with their positive effect on out of season reproductive activities,kıvırcık ewes with these genotypes might suggested to be used in out of season lambing when demanded. © 2016,veteriner fakultesi dergisi. all rights reserved.