Immune cell counts,plasma immunoglobulin contents and inf-γ gene expression in rats exposed to bisphenol A [Bisfenol A’ya maruz bırakılmış sıçanlarda ımmun hücre sayısı,plazma ımmunglobulin ıçeriği ve inf-γ gen ekspresyonu]

The purpose of this study was to evaluate the effect of low-dose graded of bisphenol a (bpa) on immune cell counts,immunoglobulin contents and gamma-interferon (inf-γ) gene expression in rats. bisphenol a was injected intraperitoneally to male wistar rats at doses of 15,30 and 45 μg/kg body weight for 5 weeks. rats were anesthetized with diethyl ether and blood samples collected,plasma separated and spleen inserted in liquid nitrogen. total and differential white blood cells,antibody titers,and gene expression of inf-γ were measured. plasma malondialdehyde levels increased linearly as bpa doses increased. bisphenol a at dose of 45 μg/kg body weight resulted in significant increase of plasma cortisol level as compared with other treatments. red blood cell counts decreased linearly as doses of bpa increased. there were no significant differences among treatment for eosinophil,monocytes and basophil counts. the gene expression of inf-γ increased as doses of bpa increased. rats received 45 μg bpa/kg body weight had 3.2 folds higher gene expression of inf-γ than the control group. the plasma igg and iga levels increased linearly as bisphenol doses increased. there was no significant difference among treatments for plasma igm level. based on the results of this study,bpa at low doses may result in increase of immune responses in a dose dependent manner. © 2016,veteriner fakultesi dergisi. all rights reserved.