Effects of BCRP and P-gp modulators on the penetration of aflatoxin B1 into the mouse brain [Aflatoksin B1’in fare beynine geçişi üzerine BCRP ve p-gp modülatörlerinin etkisi]

This study was conducted to determine whether the plasma and brain concentrations of afb1 are affected by the modulation of p-gp and bcrp using zosuquidar (zqr) and prazosin (prz),respectively. in this study,a total of 40 healthy adult male balb/c mice (32±3.7 g) were used. the animals were randomly divided into 5 groups,with 8 animals per group. group 1 was used for method validation. group 2 (af) received intraperitoneal afb1 at a dose of 20 mg/kg of body weight. groups 3 (af+prz),4 (af+zqr),and 5 (af+prz+zqr) received 20 mg/kg of afb1 intraperitoneally 30 min after the intraperitoneal administration of prazosin (0.3 mg/kg),zosuquidar (25 mg/kg),and prazosin+zosuquidar (0.3 mg/kg prazosin + 25 mg/kg zosuquidar),respectively. six hours after the administration of afb1,blood and brain samples were collected from the animals in groups 2 to 5. afb1 concentrations were determined using an hplc system with fluorescence detection. individual and simultaneous administration of prazosin and zosuquidar significantly reduced the brain concentrations of afb1 in comparison to a single administration of afb1 (p<0.05). the brain/plasma ratio of the af group was higher than that of the other groups (af+prz,af+zqr,and af+prz+zqr) (p<0.05). inducers of transmembrane proteins,especially bcrp,can be life saving during acute afb1 poisoning. © 2017,veteriner fakultesi dergisi.