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Identification,characterization and expression analysis of biglycan in Asian elephant (Elephas maximus) [Asya filinde (Elephas maximus) biglikanın tanımlanması,karakterizasyonu ve ekspresyon analizi]
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نویسنده
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chomdej s. ,saokeaw w. ,buddhachat k. ,pradit w. ,siengdee p. ,mahasawangkul s. ,sripiboon s. ,somgird c. ,nganvongpanit k. ,ongchai s. ,thitaram c.
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منبع
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journal of the faculty of veterinary medicine, kafkas university - 2017 - دوره : 23 - شماره : 6 - صفحه:997 -1001
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چکیده
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The aims of this study were to investigate the coding sequence and the deduced amino acid sequence of asian elephant’s biglycan gene as well as its expression in different tissues and conditions using wound healing as a model. the results showed that asian elephant biglycan coding sequence was 1,110 base pair (bp) long (accession number: jq753329),encoding 369 amino acids. the coding and amino acid sequences between asian and african elephants revealed 99% and 98% similarity,respectively. the conserved domains of biglycan protein were also observed. in addition,its expression was found in 15 tissues with a predominant expression in cartilage and spleen. for expression analysis in the wound healing process,it was found that the level of biglycan mrna was influenced by many factors,including age,type of wound and stage of wound healing. © 2017,veteriner fakultesi dergisi. all rights reserved.
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کلیدواژه
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Asian elephants; Biglycan; Gene expression; Sequencing; Wound healing
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آدرس
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department of biology,faculty of science,chiang mai university,chiang mai,thailand,excellent center in veterinary bioscience,chiang mai university,chiang mai, Thailand, department of biology,faculty of science,chiang mai university,chiang mai, Thailand, excellent center in veterinary bioscience,chiang mai university,chiang mai,thailand,department of biology,faculty of science,naresuan university,phitsanulok, Thailand, excellent center in veterinary bioscience,chiang mai university,chiang mai,thailand,science and technology research institute,chiang mai university,chiang mai, Thailand, excellent center in veterinary bioscience,chiang mai university,chiang mai,thailand,animal bone and joint research laboratory,department of veterinary biosciences and public health,faculty of veterinary medicine,chiang mai university,chiang mai, Thailand, national elephant institute,forest industry organization,lampang, Thailand, center of excellence in elephant,research and education,chiang mai university,chiang mai, Thailand, center of excellence in elephant,research and education,chiang mai university,chiang mai, Thailand, excellent center in veterinary bioscience,chiang mai university,chiang mai,thailand,animal bone and joint research laboratory,department of veterinary biosciences and public health,faculty of veterinary medicine,chiang mai university,chiang mai, Thailand, thailand excellence center for tissue,engineering and stem cells,center of excellence for innovation in chemistry,department of biochemistry,faculty of medicine,chiang mai university,chiang mai, Thailand, center of excellence in elephant,research and education,chiang mai university,chiang mai, Thailand
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Authors
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