Evaluation of virulence factors and phylogrouping of Escherichia coli strains isolated from acute bovine mastitis in Turkey [Akut sığır mastitislerinden izole edilen Escherichia coli suslarının filogruplandırılması ve virulens faktör değerlendirmesi]

The objective of this study was to determine the phylogenetic distribution of commonly studied virulence factors of 155 e. coli isolated from acute bovine mastitis. in the study,a,b1,b2,c,d,e,f phylogroups,and clade i were defined. b1 and c were found to be the most prevalent phylogroups covering commensal strains with a ratio of 45.2% and 37.4%,respectively. the other phylogroups determined in the study were f,d,a,and e with a range of 0.6%-8.9%. commonly known virulence genes; clpg,f17,afa,iucc,iucd,cnf1,cnf2,kps,and trat were selected to determine the virulence factors. the only virulence factor gene existing within the strains was trat (66.4%). however,any relation between phylogroups and virulence factors was not determined. phylogroups c,e and f were found to be new phylogroups for the first time in acute bovine mastitis cases. © 2017,veteriner fakultesi dergisi. all rights reserved.