Antiglycation activity of Thiamin-HCl and Benfoitiamine in diabetic condition

Objective: to observe the antiglycation effect of water-soluble vitamin, thiamin-hydrochloride (b1) and its fat-soluble derivative benfotiamine and their comparison with two different glycation assays in diabetics.methods: plasma of both the normal's and diabetic persons was taken for this experimental study which was conducted in the department of chemistry and biochemistry, university of agriculture, faisalabad in 2008. varying concentrations of both the glycation inhibitors and glucose were incubated for 5 weeks at 37°c. thiobarbituric acid and periodate borohydride assays were used to measure the antiglycation activity.results: the increase in glycation was observed from 1st to 3rd week of incubation, while it decreased after the 5th week due to the formation of advanced glycation end products.conclusions: it was observed that 10mm concentration of benfotiamine and 5mm and 1mm concentrations of thiamine-hydrochloride produced fairly good response to decreased glycation. comparison between two assays proved periodate borohydride to be more reliable and sensitive than thiobarbituric acid