Predictive accuracy of anti mullerian hormone as indicator of ovarian follicle loss in cyclophosphamide treated mice

Objective: to evaluate the strength of anti-mullerian hormone in reflecting the stages of ovarian toxicity-induced by cyclophosphamide. methods: this study was conducted in december 2014 and comprised female mice that were divided into four groups: group a served as control,group b received three weekly injections of cyclophosphamide,group c was co administered alpha-tocopherol along with cyclophosphamide,while group d solely received alpha-tocopherol. the ovaries were evaluated for follicular dynamics,and anti-mullerian hormone was assessed using mouse enzyme-linked immunosorbent assay kit. the data was analysed using spss 19. results: there were 40 mice in the study. histological analysis revealed severely reduced ovarian reserve in group b(p<0.01).in group c alpha-tocopherol conserved the ovarian reserve to near normal,thus follicle count was significantly higher than group b (p<0.05). however,this moderate reduction was still lower than the controls (p<0.01). furthermore,the number of corpus lutea and atretic follicles were significantly higher in groups b and c (p<0.01). regarding hormonal analyses in comparison to controls,anti-mullerian hormone levels were low in group b (p<0.01),while group c reported an insignificant fall in serum anti-mullerian hormone levels (p=0.101). conclusion: there was substantial evidence that anti-mullerian hormone monitoring during chemotherapy administration may fulfil the criteria of earliest diagnostic indicator of secondary infertility. © 2017,pakistan medical association. all rights reserved.