Effect of HX108-CS supplementation on exercise capacity and lactate accumulation after high-intensity exercise

Background: in the present study,we determined the effects of hx108-cs (mixed extract of schisandra chinensis and chaenomeles sinensis) supplementation on lactate accumulation and endurance capacity. furthermore,we examined ck (creatine kinase),ldh (lactate dehydrogenase) activity to determine whether the hx108-cs affected markers of skeletal muscle injury in vivo and in vitro. methods: exercise capacity was measured by an exhaustive swimming test using icr mice divided into four groups; one group received distilled water (dw) (control group,n = 10),and the other groups received three different dosages of hx108-cs (10,50 and 100 mg/kg,n = 10 per group) solution in water orally. then,for the time-dependent measurements of blood lactate,ck,and ldh,sprague-dawley rats were divided into two groups; one received dw (control group,n = 10),and the other group received hx108-cs (100 mg/kg,n = 10) solution in the same way as mice. before the exercise test,the animals were given either dw or hx108-cs for 2 weeks. high-intensity treadmill exercise was performed for 30 minutes. blood samples were collected and analyzed during and after exercise. for the in vitro experiment,c2c12 cells were treated with hx108-cs to examine its effect on lactate production,ck,and ldh activity.results: blood lactate concentration was significantly lowered immediately after treadmill exercise in hx108-cs group; however,there were no significant differences in activities of ck and ldh between hx108-cs and control during treadmill exercise and recovery phase. furthermore,treatment with 100 mg/kg of hx108-cs led to a significant increase in the time to exhaustion in swimming test,and concurrently blood lactate concentration was significantly decreased in 50 and 100 mg/kg treated group. moreover,our results of in vitro experiment showed that hx108-cs suppressed lactate production,ck,and ldh activity in a dose-dependent manner. conclusions: these results suggest that supplementation with hx108-cs may enhance exercise capacity by lowering lactate accumulation. this may in part be related to an amelioration of skeletal muscle injury. © 2012 oh et al.; licensee biomed central ltd.