Studies on the role of tumor necrosis factor- alpha (TNF-α) in hepatocytes induced apoptosis in vaccinated,Schistosoma mansoni-challenged mice

Tumour necrosis factor-alpha (tnf-α) plays a complex role in pathophysiological changes caused by schistosomiasis in the liver cells as induced apoptosis. so,the highlighted experimentally the role of tnf-α in hepatocytes apoptosis,using that as an assessment of the efficacy of antischistosomal vaccination by mixed crude antigens preparations [cercarial antigen preparation (cap) + soluble worm antigen preparation (swap) + soluble egg antigen(sea)] by parasitological,histo-pathological and histochemical studies using feulgen stain of hepatoytes dna,a serological study also of serum tnf-α level by elisa. fifty two laboratory bred albino male mice,were used in this study. they were classified into four groups (13 mice in each group),g1: normal control,g2 as infected control while g3 supported by freund's adjuvant (f. adj) then infected and g4 vaccinated with combined antigens (cap,swap and sea) + f. adj,then infected. mice were sacrificed by cervical dislocation 9 weeks post infection,parasitological (kato-katz thick smear for egg count),histopathologial {haematoxylin and eosin (h&e) staining of hepatic sections},histochemical (feulgen staining of hepatocytes dna) and elisa to estimate serum tnf-α level were performed. the data showed that vaccination with combined antigens showed protective effect on vaccinated then schistosoma challenged mice,hepatocytes induced apoptosis was directly proportional with the tnf-α serum level,and the protection degree of potential combined vaccine was inversely proportional with serum tnf-α level and induced apoptosis.