Determination of amino acids in industrial effluents contaminated soil

38 samples of soil for 19 locations partially irrigated on the effluents of sugar mill and oil andghee mill,bottom sediments of evaporation ponds of sugar and fertilizer industries were collected and analyzed for amino acids after acid digestion by gas chromatography using pre column derivatization with trifluroacetyleacetone and ethyl chloroformate. the results obtained were compared with the soil samples irrigated with fresh water. the soil samples were also analyzed for ph,total nitrogen contents and organic carbon. nine essential {leucine (leu),threonine (thr),lysine (lys),l-phenylalanine (phe),tryptophan (trp),histadine (his),l-valine (val),methionine (met) and isoleucine ile) and ten non-essential ( alanine (ala),cysteine (cys),asparagine (asn),glutamic acid (glu),serine (ser),glycine (gly),proline (pro),glutamine (gln),aspartic acid (asp),tyrosine (tyr)} amino acids were analyzed 13..15 amino acids were identified and determined quantitatively from soil samples. amino acids met,asn,gln and trp were observed absent from all the samples. the variation in the amino acids contents in soil with the industrial effluents added and total nitrogen and organic carbon is discussed.