Effect of streptolysin S on the luminol-amplified chemiluminescence response of rabbit polymorphonuclear leucocytes

The effects of purified streptolysin s (sls) produced by streptococcus pyogenes group a on polymorphonuclear leucocytes (pml) was investigated. the activation of the oxidative metabolism was determined by chemiluminescence amplified with luminol. purified sls,at concentrations of between 8000 hu/ml and 256 hu/ml,when added directly to n-formylmethionyl-leucyl-phenylalanine (fmlp)-induced rabbit pml,caused inhibition of the pml chemiluminescence response. pml without added fmlp (i.e. not induced),served as background controls,and gave no significant endogenous cl response compared to cells induced with fmlp,which served as positive controls. inhibition of the chemiluminescence response could be the result of a lethal effect of sls on pml or it could reflect a more specific effect of the toxin on the membrane-mediated chemiluminescent response. © 2015,chemical society of pakistan. all rights reserved.