Cyanoacetamide based barbiturates,thiobarbiturates and their biological studies

Various cyanoacetamide based knoevenagel adducts were coupled with barbituric acid/thiobarbituric acid and triethylorthoformate via a one pot three component reaction in 2-butanol availing the desired compound in excellent yields. all the synthesized compounds (2-15) were extensively characterized by 1h-nmr,13c-nmr,mass spectrometry and elemental analysis and were screened for antibacterial,antiurease,antioxidant,cytotoxicity and chymotrypsin inhibition studies. in case of antibacterial studies 2 was found appreciably active against the six selected strains whereas the rest of the compounds were moderately active. the urease inhibition studies revealed compound 5 and 12 as potent whereas the rest were found inactive where thiourea was used as control. antioxidant activity results exhibited with 2 as the most active and rest of compounds showed good activity. in case of chymotrypsin inhibition studies all the synthesized compounds were found inactive with the exception of 6 which was moderately active.