Urease inhibition of fixed oils and fractions of Caralluma tuberculata: Component identification by GC-MS

The in vitro urease inhibitory activity of fixed oil and various organic fractions of caralluma tuberculata followed by gc-ms analysis of the fixed oil are described in this research article. the fixed oil caused marked attenuation of jack bean urease with half-maximal inhibitory concentration (ic50) of 2.97 mg/ml. the similar urease inhibitory profile was observed for chloroform and ethyl acetate fractions with ic50 values of 3.36 and 4.90 mg/ml,respectively. gcms analysis led to the identification of 20 different constituents of the fixed oil by their m/z ratio and retention time in comparison with the standard compounds. the major constituents were methyl linoleate (30.97%) followed by methyl octadecadienoate (19.16%),ethyl linolenate (13.70 %),and methyl palmitate (9.86 %). the fixed oil and organic fractions of c. tuberculata exhibited marked urease inhibition and thus provide scientific background for use of the plant as antiulcer agent.