Synthesis and chemical characterization and DNA binding of ferrocene based ureas

This article reports synthesis and characterization of 1-(4-ferrocenylphenyl)-3-(6-(3-(4-ferrocenylphenyl)ureido)hexyl)urea (1),1-(3-ferrocenylphenyl)-3-(6-(3-(3-ferrocenylphenyl)ureido)hexyl)urea (2) and 1-(3-ferrocenylphenyl)-3-(6-(3-(3- ferrocenylphenyl)ureido)-2-methylphenyl)urea (3) and their binding with dna binding. structural characterization was accomplished by ft-ir,multinuclear (1h and 13c) nmr spectroscopy and melting point. cyclic voltammetric measurements were used to probe electrochemical behavior,mode of interaction and dna binding potency with parameters 1-3. in voltammogram,a shift in peak potential and drop in peak current was observed with increasing concentration of ct-dna. the negative shift in peak potential indicated electrostatic interactions for 1-3 with negatively charged phosphate of dna backbone. the binding constant,binding free energy,binding ratio werecalculated and found very impressive. the high negative value of -δg signifies the spontaneity and high conformational stability of the compounds with dna.