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   Development and validation of reversed-phase high performance liquid chromatographic method for analysis of cephradine in human plasma samples  
   
نویسنده ahmad m. ,usman m. ,madni a. ,akhtar n. ,khalid n. ,asghar w. ,bakhsh s.
منبع journal of the chemical society of pakistan - 2010 - دوره : 32 - شماره : 1 - صفحه:58 -63
چکیده    An hplc method with high precision,accuracy and selectivity was developed and validated for the assessment of cephradine in human plasma samples. the extraction procedure was simple and accurate with single step followed by direct injection of sample into hplc system. the extracted cephradine in spiked human plasma was separated and quantitated using reversed phase c 18 column and uv detection wavelength of 254 nm. the optimized mobile phase of new composition of 0.05 m potassium dihydrogen phosphate (ph 3.4)-acetonitrile (88:12) was pumped at an optimum flow rate of i ml.min -1. the method resulted linearity in the concentration range 0.15-20 μg.ml -1 the limit of detection (lod) and limit of quantification (loq) were 0.05 and 0.150 μg.ml -1. respectively. the acc0uracy of method was 98.68 %. this method can be applied for bioequivalence studies and therapeutic drug monitoring as well as for the routine analysis of cephradine.
آدرس department of pharmacy,faculty of pharmacy and alternative medicine,islamia university, Pakistan, department of pharmacy,faculty of pharmacy and alternative medicine,islamia university, Pakistan, department of pharmacy,faculty of pharmacy and alternative medicine,islamia university, Pakistan, department of pharmacy,faculty of pharmacy and alternative medicine,islamia university, Pakistan, department of pharmacy,faculty of pharmacy and alternative medicine,islamia university, Pakistan, department of pharmacy,faculty of pharmacy and alternative medicine,islamia university, Pakistan, faculty of pharmacy,gomal university, Pakistan
 
     
   
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