Gas chromatographic analysis of tranexamic acid in pharmaceutical preparation using ethyl chloroformate as derivatizing reagent

Ethyl chloroformate (ecf) has been used as derivatizing agent for gas chromatographic (gc) determination of tranexamic acid (ta). the elution was from the column hp-5 (30 m x 0.32 mm i.d.) connected with flame ionization detection (fid). linear calibration curves were obtained with 2-50 ng ta injection on the column. aminocaproic acid (aca) when present together with ta could also be separated and determined simultaneously. ta was determined from pharmaceutical preparation. relative standard deviation (rsd) for analysis was observed within 0.1-0.4%. the pharmaceutical additives and number of amino acid when present together with ta did not affect the determination of ta.