Capillary gas chromatographic determination of dopamine by pre-column derivatization method using trifluoroacetylacetone as derivatizing reagent

Capillary gas chromatographic separation of putrescine (pu),cadaverine (cd),dopamine (da) and octopamine (oa) as derivatives of trifluoroacetylacetone (faa) has been carried out using a column hp-5 (30 m × 0.32 mm id),temperature 100 °c for 1 min,followed by heating rate 10 °c/min up to 250 °c using fid detection. nitrogen flow rate was 4 ml/min and split ratio was 10:1 v/v. the linear calibration graphs were ranging between 2.5-25 μg/ml. the method was applied for the determination dopamine in pharmaceutical preparations with relative standard deviation (rsd) within 1.5-2.5% and recovery of 98% with rsd 1%.