Preparation of bovine serum albumin hollow microparticles by the water-in-oil emulsion solvent diffusion technique for drug delivery applications

Biodegradable bovine serum albumin (bsa) hollow microparticles have been prepared by a single step and rapid water-in-oil emulsion solvent diffusion method without any emulsifiers and templates. aqueous bsa solution and ethyl acetate were used as water and oil phases,respectively. bsa solution was cross-linked with polyethylene glycol diglycidyl ether (pegde) before microparticle formation. methylene blue (mb) was used as a water-soluble model drug to entrap in the microparticle matrix. the non-cross-linked and cross-linked bsa microparticles contained empty core structure with outer smooth surface. inner surface and matrix of hollow microparticles consisted void structure. drug loading did not affect the microparticle morphology. cumulative drug released from microparticles was decreased steadily as decreasing of mb ratio and increasing of pegde ratio. the bsa hollow microparticles may have potential application in controlled release drug delivery application.