Physiological and biochemical constituents as predictive appreciation for selection of drought tolerant cultivars in wheat (Triticum aestivum L.)

A pot study was undertaken to assess the effect of drought stress imposed at various growth stages on growth,physiological and biochemical attributes of wheat. five commercial wheat cultivars viz. chakwal-97,inqalab-91,margalla-99,nr-234 and wafaq-2001 were grown in pots. the plants were subjected to three consecutive drought cycles at tillering,pre-anthesis and milky growth stages. measurements pertaining to various physiological and biochemical parameters such as relative water content (rwc),proline,superoxide dismutase (sod),membrane stability index (msi),yield and yield components were made. significant reduction in grain yield was observed in all the test varieties when drought was imposed at any growth stage. the reduction was highest (39- 64%) when stress was imposed at pre-anthesis followed by tillering growth stage. the wheat variety wafaq-2001 and inqalab-91 performed better by giving higher yield and produced greater numbers of filled seeds per spike compared to other varieties. under water stress proline contents were higher in the drought-tolerant cultivar wafaq-2001. the same variety showed higher membrane stability index and antioxidant enzymes (sod) activity under drought stress conditions. the results suggest that pre-anthesis growth stage is the most sensitive towards drought stress. wheat cultivars: wafaq- 2001 and inqalab-91 showed best tolerance response against drought stress. higher proline,rwc and sod activity under drought stress seems to be the most reliable parameters enabling the discrimination of varieties for drought tolerance.