Determination of glucuronic acid and glucosamine in the capsule polysaccharides from streptococcus equi subsp. Zooepidemicus by HPLC

Partially purified capsule polysaccharides fraction (pcp) were obtained from the culture of streptococcus equi subsp. zooepidemicus c55129 by precipitation using 75% ethyl alcohol and through an anion-exchange column chromatography eluted by nacl. the main component of pcp was hyaluronic acid. a simple method for the determination of glucuronic acid and glucosamine in pcp by high-performance liquid chromatography (hplc) with refractive index detector was developed and validated. the chromatographic separation was achieved with a shodex sugar ks-801 column and a sugar-d column after pcp was hydrolysed by trifluoroacetic acid and hydrochloric acid,respectively. the method showed good linearity,sensitivity,precision and recovery,and proved very simple and rapid for routine analysis since separations were completely achieved.