Development and validation of spectrophotometric method for the determination of nimesulide in bulk and tablet dosage forms by biuret reagent method

The purpose of the study was to develop a method for the determination of nimesulide using visible spectrophotometry. it is based on the reaction of nimesulide with biuret reagent,which forms a greenish coloured complex. the formed complex exhibited maximum absorption at 400 nm. the limit of detection of nimesulide was 2.5 μg ml -1 and the limit of quantification was 5.0 μg ml- 1. the developed method has acceptable accuracy (97-101%) and correlation coefficient (< 3%). the method was successfully applied on three different tablet dosage brands available in pakistani market and statistical data shows that results are reliable and reproducible.