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Antibacterial activity of vacuum liquid chromatography (vlc) isolated fractions of chloroform extracts of seeds of achyranthes aspera
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نویسنده
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noor-ul-amin college of pharmacy ,qadir m.i. ,khan t.j. ,abbas g. ,ahmad b. ,hussain k.j. ,ali m.
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منبع
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journal of the chemical society of pakistan - 2012 - دوره : 34 - شماره : 3 - صفحه:589 -592
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چکیده
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Antibacterial activities of locally occurring weed achyranthes aspera were studied. three solvents (hexane,chloroform,and ethanol) were used successively for the extraction of active principles from the seeds of this plant. the extracts were concentrated on vacuum rotary evaporator. the concentrated extracts were tested for their antibacterial activities after making their solution in gum acacia. the six bacterial strains used in the antibacterial studies were bacillus subtilis,micrococcus luteus,staphylococcus aureus,escherichia coli,pseudomonas aeuroginosa,and salmonella chloerasuis. antibacterial activities of the extracts were compared with streptomycin and ampicillin in terms of zones of inhibition. chloroform and ethanol extracts demonstrated antibacterial activity. hexane extract did not demonstrate antibacterial activity. chloroform extract was more potent than alcohol extract in terms of antibacterial activity. an attempt was made to identify the nature of compound by isolation through vacuum liquid chromatography (vlc). the fractions isolated by vlc were subjected to thin layer chromatography (tlc). tlc showed the presence of alkaloids and terpenoids. the active fractions were tested for their antibacterial activity. one of the fractions exhibited antibacterial activity.
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کلیدواژه
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Achyranthes aspera; Antibacterial activity; Isolated fractions; Vacuum liquid chromatography
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آدرس
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government college university, Pakistan, college of pharmacy,government college university, Pakistan, university college of pharmacy,university of the punjab, Pakistan, college of pharmacy,government college university, Pakistan, faculty of pharmacy,bahauddin zakariya university, Pakistan, faculty of pharmacy,bahauddin zakariya university, Pakistan, institute of biotechnology,bahauddin zakariya university, Pakistan
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Authors
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