Ultrasonically assisted extraction and hplc determination of chondroitin sulfate from fish heads

In the present study,we investigated the ultrasonically assisted extraction(uae) and high-performance liquid chromatography (hplc) determination of chondroitin sulfate(cs) from fish heads. firstly,the optimized parameters for cs extraction were obtained by using box- behnken design(bbd) and response surface methodology(rsm) based on the single-factor experiments. as results,the optimum conditions were ultrasonic time 45min,ultrasonic power 280w and solvent/material ratio 6ml/g. under these conditions,the experimental yield of cs was 4.623%. secondly,the cs was purified by deae-cellulose 52 chromatography and sephadex g- 100 chromatography to afford purified cs. finally,we developed a simple method for the determination of glucuronic acid (glca) and n-acetylgalactosamine (galnac) in purified cs by hplc with a diode array detector (dad). the results showed that there were obvious saccharides peaks in cs.