Antioxidant,antimicrobial,cytotoxic studies of methanolic extract,fractions and essential oil of curry patta (Chalcas koeingii) from Pakistan

The present study was carried out to examine the antioxidant antimicrobial activities and cytotoxicity of methanolic extract and essential oil of chalcas koeingii leaves. the ground leaves were extracted with absolute methanol and further fractionated by solvent-solvent extraction method with increasing polarity based absolute solvents i.e. n-hexane,chloroform,ethyl acetate and nbutanol. the chalcas koeingii leaves extract and fractions contained appreciable levels of total phenolic contents (99.82 -1750.23 ce,mg/100g) and total flavonoid contents (70.23 -1159.23 gae,mg/100g). the gc-ms analysis of chalcas koeingii essential oil revealed the presence of 19 compounds,with 3,7,11,15-tetramethyl-2-hexadecane(19.76 %),crocetane (13.28 %),benzene,1,1- [thiobis(methylene)]bis[4-chloro (12.77 %),2-chloro-1,2-diphenylethanone (9.24 %),hexatriacontane (6.99 %) as the major components. chalcas koeingii leaves extracts also exhibited good dpph radical scavenging activity,showing ic50 ranged from 17.37-84.23μg/ml and % inhibition linoleic acid peroxidation 37.23-91.09 respectively. the results of the present study demonstrated significant (p<0.05) variations in the antioxidant activities of chalcas koeingii leaves essential oil,extract and fractions.