Qualitative and quantitative assessment of fatty acids of buddleja asiatica by GC-MS

To analyze the fatty acid contents of buddleja asiatica lour,both the non-volatile oil and fat obtained from the n-hexane soluble sub- fraction were subjected to gc/ms using bstfa (n,obis(trimethylsilyl) trifloroacetamide) derivatization. the oil showed the presence of six fatty acids including palmitic acid (46.75 %),linoleic acid (37.80 %),stearic acid (10.98 %),arachidic acid,margaric acid and lignoceric acid (< 3 %) . analysis of the fat revealed nine fatty acids including lignoceric acid (43.12 %),behenic acid (26.39 %),arachidic acid (9.29 %) and stearic acid (5.3 %). cerotic acid,montanic acid,melissic acid and palmitic acid were found in low amounts (< 5 %) while trycosylic acid (4.83 %) was the only fatty acid with odd number of carbon atoms. the oil showed a low thermal stability.